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KMID : 1161420110140111419
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Journal of Medicinal Food
2011 Volume.14 No. 11 p.1419 ~ p.1424
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Phenolic Composition and Antimicrobial and Antioxidant Activities of Leucoagaricus leucothites (Vittad.) Wasser
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Aslim Belma
Ozturk Sahlan
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Abstract
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Leucoagaricus leucothites (Vittad.) Wasser is an edible macrofungus found limitedly in Turkey. The ethanolic extract of L. leucothites has shown strong antimicrobial activity against some foodborne and spoilage bacteria. Therefore, the phenolic composition of L. leucothites ethanolic extract was analyzed by using high-performance liquid chromatography. Catechin (288?mg/L) was found to be the major component in the extract. Antioxidant activity of the extract was evaluated by studying the scavenging ability of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, and chelating ability of the extract was assessed in a ferrous ion assay. The scavenging effect on DPPH radicals was 71% at 10?mg/mL, whereas the chelating effect on ferrous ions was 82% at 15?mg/mL. The total contents of phenol (3.40±0.5?mg/g), ascorbic acid (2.10±0.3?mg/g), β-carotene (0.02±0.0?mg/g), and lycopene (0.01±0.0?mg/g) in the macrofungi ethanolic extract were also determined.
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KEYWORD
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antimicrobial activity, antioxidant activity, high-performance liquid chromatography analysis, Leucoagaricus leucothites
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